Composite

Part:BBa_K2967023:Design

Designed by: Lijun Bao   Group: iGEM19_NEU_CHINA   (2019-10-02)


PytfE-RBS-Luc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 194
    Illegal NgoMIV site found at 1538
    Illegal NgoMIV site found at 1559
    Illegal AgeI site found at 1262
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1444


Design Notes

This part can be used to detect the nitric oxide.

Source

The promoter PytfE comes from the genome of K12 strain.

References

Justino, M.C., et al., Escherichia coli YtfE is a di-iron protein with an important function in assembly of iron-sulphur clusters. FEMS Microbiol Lett, 2006. 257(2): p. 278-84.